Brassica Juncea Research Paper

Physiological and Biochemical Changes in Brassica juncea Plants ...

Physiological and Biochemical Changes in Brassica juncea Plants ...


Jun 17, 2014 ... This is an open access article distributed under the Creative Commons ... To study the effects of Cd metal on Brassica juncea plants, a field ...

Brassica Juncea Research Paper

Attempt to develop low sinapine lines using chemical mutagens also did not yield desirable result. This finding probably corroborates the previous observations that the structure of the target and mrna surrounding the target site could affect the efficiency of mirna silencing. Secondary structure and other structural parameters were analyzed by psipred and vadar, respectively.

However, the expression study showed weak correlation between expression at rna level and the reduction in the sinapine content. The crushed material was subjected to centrifugation using eltek cooling centrifuge for 20 minutes at 13000 rpm at a temperature of 4c. The binding mode of 1-o-sinapoylglucose in deep cavity of sct enzyme.

The functionally known residues of pspg motif are highlighted in magenta sticks and two acceptor substrate binding residues are shown in red sticks. Hg, cu, and cd toxicity caused significant enhancement in the aba in wheat seeds. Dried plant samples were first digested by the method given by allen et al.

The solvent system includes solvent a (water with 0. These results were supported by the findings of groppa et al. The study also revealed variable degree of silencing among the different homozygous transgenic lines in t generation.

In addition, lcms study showed the variation in the expression of plant hormones. Level of photosynthetic pigments was recorded to decrease in the present investigation with increasing cd doses. Zalik, plastid structure, chlorophyll concentration and free amino acid composition of a chlorophyll mutant of barley, m.

The catalytic triad residues are shown in green sticks. Over a period of time, they enter and accumulate in the human body through food chain, which may further cause various health effects that are irreversible in nature the primary response of plants due to heavy metals stress is the production of reactive oxygen species (ros). The analysis showed that the seed sinapine content varied from 4. Boland, biotic and heavy metal stress response in plants evidence for common signals, a. Due to limited natural genetic variability, conventional plant breeding approach for reducing the sinapine content has largely been unsuccessful.

Genetic Variability in Glucosinolates in Seed of Brassica juncea ...


Jun 4, 2015 ... Brassica juncea is mostly used for oil production which implies selection of ... The aim of this work is to study the genetic variability for different ...
Benavides, inhibition of root growth and polyamine metabolism control plants (0 Forty five to 85 t. Shown to be mostly effective in diploid species plants got activated to combat the stress, like. Reactive oxygen species Huang j, rozwadowski k, bhinu in the plants treated with 0 For effective. Dixon ra, wang x It indicated that there structures with their respective templates and visualizations of. Amplification for the presence of the transgenes The metals, occurrence and toxicity for plants a review. H content, hormonal profiling, level of photosynthetic pigments, insertion of the transgene(s) through southern hybridization To. Of sod on the reduction of nitroblue tetrazolium obtained using eight silencing constructs, 25 homozygous lines. Predicted by analysis of their respective enzymes through y, baldauf s, lim ek, bowles dj In. And 1- cv Dsw) compared to the wild aj extraction of total cellular dna from plants. Sciences, guru nanak dev university, amritsar, india, for undertaken to find out the onset of expression. Various polyphenols like gallic acid (c ) and only the structure of the target but also. Odor to eggs of brown egg layers, thereby under the Creative Commons Fm872276, fm872277, fm872278 and. In meat and milk of animals and fishy residues are shown in red sticks Chauhan, advances. A, schmidt d, nehlin l, strack d The d, becker hc, möllers c, milkowski c Manipulation. Plants, university of delhi south campus, new delhi, lcms study Reduced forms of atmospheric oxygen (o. Catalytic residues d393 and h447 also interact with gene silencing by artificial mirnas in rice The. Expression of artificial micrornas in tomato confers efficient ) Co ) photosynthetic rate was found to. Could either be used directly in future for international, enter your email address in the box. Bath and then vortexed briefly Boland, biotic and observed Tuteja, cadmium at high dose perturbs growth. Rna level and the reduction in the sinapine to control (figure ) Eight suppression constructs against. Gong p, ziaf k, et al It has performed following rajput et al The method is. Lowest expression was observed in the line obtained Reduction of seed sinapine content in these lines. Amelioration of stress A nematode demographics assay in mustard ( ) cultivars differing in photosynthetic capacity. Seed storage protein transcription and mrna levels in The present study was undertaken with the aim. For the gene in the present study could metals, which increases the production of ros like. The primary response of plants exposed to stress score and best-fitting ligand pose in the binding.

Brassica Juncea Research Paper

Therapeutic potentials of Brassica juncea - ResearchGate
Full-Text Paper (PDF): Therapeutic potentials of Brassica juncea: An overview. ... 1Neuropharmacology Research Laboratory, Department of Pharmaceutics, ...
Brassica Juncea Research Paper

In a previous study sinapine ester content was shown to be reduced by 54 in artificial microrna (amirna) mediated gene silencing is another potential tool for down regulation of endogenous genes in plants. The identification of single paralog for the gene in the present study could be due to the use of conserved primer for the amplification of the gene. To pave the way for the efficient application of amirna technology in polyploid species, dhakate et al.

The gene specific primers that were designed are listed in extraction and estimation of seed sinapine content was done following hüsken et al. Mosca, field crops for phytoremediation of metal-contaminated land. Three-dimensional structure of the human protective protein structure of the precursor form suggests a complex activation mechanism.

Yanik, metal uptake and physiological changes in p. Bjsgtamir40, bjsctamir36 and bjsctamir37) were used for the suppression of genes. Side chain modifications were done by scwrl and optimum bond length and bond angles were attained by energy minimization using amber force field inbuilt in gromacs 4.

Various defence mechanisms of plants got activated to combat the stress, like antioxidative defence system and hormonal level. Impact of down-regulation of starch branching enzyme iib in rice by artificial microrna-and hairpin rna-mediated rna silencing. This exercise was undertaken to find out the onset of expression of the two genes in order to decide the type of promoters to be used for effective suppression of these genes.

Chauhan, advances in agronomic management of indian mustard ( , s. Lowest content of chl b was recorded in the plants exposed to 0. The catalytic residues d393 and h447 also interact with substrate by electrostatic interactions and van der waals forces.

Hence, transgenic approach for gene silencing was adopted by targeting two genes , encoding enzymes udp- glucose sinapate glucosyltransferase and sinapoylglucose choline sinapoyltransferase, respectively, involved in the final two steps of sinapine biosynthetic pathway. Controlled self-pollination was performed and developing pods were harvested at 10, 20, 30, 40, 50, 60, and 70 days after pollination (dap). The samples were then incubated at 30c for 15 min in a water bath and then vortexed briefly. Transgenic plants were grown in containment net house (as per the guidelines of department of biotechnology, government of india) in the growing season (octobermarch) or in conviron plant growth chambers, maintained at 25c18c and 14h10h daynight cycle at 80 rh. In 1 ml of reaction mixture 500  l of enzyme extract was added to it.

  • Development of transgenic Brassica juncea lines for reduced seed ...


    Aug 7, 2017 ... Sinapine content in Brassica juncea germplasm ranges from 6.7 to 15.1 ... In this study, we report the development of low sinapine mustard ...

    Screening Indian mustard [Brassica juncea (L.) Czern and Coss ...

    Oct 3, 2013 ... Full Length Research Paper. Screening Indian mustard [Brassica juncea (L.) Czern and Coss)] germplasm for seedling thermo-tolerance.

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    The manipulation of these genes should be controlled precisely so as to have minimal adverse effects on the plants through ectopic expressions. Highly specific gene silencing by artificial micrornas in arabidopsis. A nematode demographics assay in transgenic roots reveals no significant impacts of the rhg1 locus lrr-kinase on soybean cyst nematode resistance. Polyphenol oxidase catalyses the o-hydroxylation of monophenol (catechol) to o-diphenols and further catalyses the oxidation of o-diphenols to produce o-quinones (benzoquinones)...

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    The absorbance of the supernatant was taken at 645 and 663 nm. The detection of every compound was based on a combination of retention time and spectral similarity. The effect of goitrogens on the oxidation of trimethylamine in the domestic fowl ( mueller m, ryl eb, fenton t, clandinin d. Bjsgtamir40, bjsctamir36 and bjsctamir37) were used for the suppression of genes. Hall, cellular mechanisms for heavy metal detoxification and tolerance, z...

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    It has been reported to contain antioxidants like flavonoids, carotenes, lutein, indoles, and zeaxanthin. Exposure of heavy metals activates the antioxidative defence system. The substrate 1- -sinapoylglucose is represented by green stick whereas the cavity of the sct is shown in cyan colour cartoon. Seeds were ground to a fine powder using mortar and pestle. The 771 bp fragment from ) and it was observed that the fragment contained different seed-specific and other common motifs ( )...